石决明对大鼠白内障晶状体中SOD和GSH及GSH-PX的影响

Effect of shijueming on SOD,GSH and GSH-PX in cataract lens of rats

目的:探讨石决明对白内障大鼠氧化损伤的保护机制,为寻求有效的防治白内障药物提供可靠的实验依据。方法:将80只大鼠随机分为4组,每组20只,制作D-半乳糖性白内障大鼠模型后,空白组和模型组分别点生理盐水,白内停组给白内停滴眼液点眼,石决明组给石决明水提液。于实验开始后第5,10,15d散瞳,在裂隙灯下观察晶状体的变化并摄影。之后处死大鼠,取出晶状体,测定超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)含量。结果:空白组SOD,GSH和GSH-PX含量明显高于其它三组(P<0.01);石决明组SOD,GSH和GSH-PX含量高于模型组,有明显差异(P<0.05);石决明组与模型组比较,石决明组大鼠晶状体混浊程度明显轻,发展缓慢;也略优于白内停组。结论:石决明中药水提液对白内障大鼠的晶状体有保护作用,可延缓白内障的发展。

AIM： To investigate the mechanism of shijueming prevention and cure on cataract METHODS： Eighty rats were randomly divided into 4 groups,with 20 in each group.Ten animals in group A were taken as health control,the other animals in group B、C and D were made as D-galactose cataract models.Group A and group B were dropped with saline eyedrops;Group C was dropped with pirenoxine sodium eyedrops;Group D was dropped with extract liquid from shijueming.Then,the animals＇ pupils were dilated and lens changes were observed under the slit-lamp and taken photograph at 5th,10th,15th day.At the 15th day all animals were killed,the content of SOD,GSH and GSH-PX from lens were measured·RESULTS： The content of SOD,GSH and GSH-PX was significantly higher in the group A than that in group B,C and D（P〈0.01）;The content of SOD,GSH and GSH-PX was significantly higher in the group D than that in group B（P〈0.05）.The degree of lens opacity in rats： compared with group B,group D had significantly lighter lens opacities and slower development,also slightly better than group C·CONCLUSION： Shijueming can effectively improve the antioxidant capacity of the lens,and protect the lens against cataract development.