N-乙酰半胱氨酸对紫外线损伤大鼠晶状体水通道蛋白-1的影响

Expression of aquaporin protein-1 on rat lens after ultraviolet radiation injury and the effects of N-acetylcysteine on it

目的:观察N-乙酰-L-半胱氨酸(N-acetylcysteine,NAC)对紫外线(ultraviolet,UV)损伤的大鼠晶状体水通道蛋白-1(aquaporin protein-1,AQP-1)表达的影响。方法:将健康清洁级SD大鼠的离体透明晶状体随机分为3组,模型组用UV照射,药物组除照射外另加入NAC,对照组均不采取以上处理。分别培养3,12,24h后观察大鼠晶状体的混浊程度和水化程度;Western-blot法观察AQP-1的表达情况。结果:对照组晶状体高水平表达AQP-1;模型组晶状体低表达AQP-1;药物组较模型组表达较高AQP-1。经统计学分析,模型组与对照组之间AQP-1的表达有显著性差异(P<0.01),模型组和药物组之间AQP-1的表达有显著性差异(3h时P<0.05,12和24h时P<0.01)。结论:UV照射能降低AQP-1的表达,导致白内障,而NAC能通过提高AQP-1的表达保护细胞免受UV损伤,从而减缓白内障的发生。

AIM ：To investigate the effect of N-acetylcysteine（NAC） on the rat lens expression of aquaporin protein-1（AQP-1） during ultraviolet（UV） radiation injury·METHODS： Transparent lens of healthy clean grade SD rats were randomly divided into 3 groups,the model group was exposed with UV ray,the drug group added in 300μmol/L NAC besides the UV radiation,the control group did not take above measures.Respectively they were cultivated 3,12 and 24 hours to observe the degree of lens opacification and the lens hydration degree;Western blotting was used to observe the levels of AQP-1 expression·RESULTS：The control group had high expression of AQP-1;the model group low expression of AQP-1;compared with model group,the drug group had high expression of AQP-1.By statistical analysis,between the model group and the control group,the expression of AQP-1 had significant difference（P〈0.01）;between the model group and the drug group,the expression of AQP-1 also had significant difference（at 3 hours,P〈0.05;while at 12,24 hours,P〈0.01）·CONCLUSION： UV ray can reduce the expression of AQP-1 and lead to cataract,while NAC can protect cells from UV damage to ease cataract incidence by improving the expression of AQP-1.