血清中VEGF和bFGF水平与糖尿病视网膜病变关系的临床研究

Relationship between serum level of the basic fibroblast growth factor and vascular endothelial growth factor and proliferative diabetic retinopathy

目的:探讨糖尿病视网膜病变患者血清中血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)水平与糖尿病视网膜病变的关系。方法:收集患有糖尿病视网膜病变白内障患者的血清样本,并以无糖尿病白内障患者的血清样本作为对照,用ELISA方法测定血清中bFGF,VEGF的含量。结果:对照组12例血清样本bFGF含量为(34.95±9.62)ng/L,VEGF含量为(150±20)μg/L;单纯型DR组中10例bFGF含量为(52.6±7.54)ng/L,VEGF含量为(330±50)μg/L;增生型DR组7例bFGF含量为(64.929±11.917)ng/L,VEGF含量为(580±50)μg/L。和对照组相比糖尿病患者血清中bFGF,VEGF的含量均显著增加(P<0.01),并且随着DR病情的进展而增高(P<0.05);血清中VEGF与bFGF之间呈正相关(r=0.419,P<0.01)。结论:VEGF,bFGF参与了DR的发生发展,并与DR后期新生血管形成关系密切。

AIM： To explore the changes of the serum level of basic fibroblast growth factor（bFGF）,vascular endothelial growth factor（VEGF） in diabetic patients and to investigate the role of bFGF,VEGF in the pathogenesis of diabetic retinopathy（DR）·METHODS：Serum samples of DR patients with cataract were collected.The serum samples from the non-diabetic patients with cataract were chosen as the control.The serum level of the bFGF,VEGF protein was analysed by ELISA method·RESULTS： The levels of serum VEGF and bFGF were significantly higher in patients with DR than those in controls（P〈0.01）;The serum levels of VEGF and bFGF in patients with proliferative diabetic retinopathy（PDR） were significantly higher than those in non-PDR patients（P〈0.05）.The serum levels of VEGF and bFGF were associated with the severity degree of DR.Positive correlations were found between bFGF and VEGF（r=0.419,P〈0.01）·CONCLUSION： bFGF and VEGF play an active role in the generation and development of DR and has a close relation with the neovascularization of the advanced stage of DR.