内皮素、一氧化氮、烫伤血清对大鼠心脏微血管内皮细胞ICAM－1表达的影响

The effect of endothelin,nitric oxide and burned rat serum on the ICAM-1 expression of rat cardiac microvascular endothelial cells

目的：观察内皮素（ＥＴ）、一氧化氮（ＮＯ）合成酶抑制剂Ｌ－ＮＡＭＥ以及烫伤血清对心脏微血管内皮细胞细胞间粘附因子ＩＣＡＭ－１表达的影响。方法：分离、培养的ＳＤ大鼠心脏微血管内皮细胞分别用ＥＴ、Ｌ－ＮＡＭＥ、烫伤血清刺激后ＥＬＩＳＡ测定内皮细胞ＩＣＡＭ－１的表达。结果：ＥＴ、Ｌ－ＮＡＭＥ、烫伤血清均能明显诱导微血管内皮细胞ＩＣＡＭ－１的表达。ＥＴ在刺激后２ｈ达到高峰，是正常对照的４．５５倍；Ｌ－ＮＡＭＥ刺激后的表达高峰在１ｈ，是正常的３倍。烫伤血清刺激后ＩＣＡＭ－１在４ｈ后表达最强，为正常的３．５倍。结论：烫伤血清、ＥＴ、ＮＯ均参与、介导大鼠心脏微血管内皮细胞ＩＣＡＭ－１的表达，但表达高峰不在同一时相。

Objective:To observe the effect of endothelin(ET),nitric oxide (NO) and burned rat serum on the ICAM-1 expression of rat cardiac microvascular endothelial cells.Methods:The ICAM-1 expression was measured using ELISA after the endothelial cells were incubated with ET,L-NAME and burned rat serum respectively in different time. Results:The ICAM-1 expression increased significantly after stimulation.The most dramatic rise of ICAM-1 expression was at 2h,1h and 4h after incubation with ET.LNAME and burned rat serum respectively, which was 4.55,3, 3. 5 times as much as control.Conclusion:ET,NO might play a role in the ICAM-1 expression of rat cardiac microvascular endothelial cells which might participate in the injury of endothelial cells after burns.