精氨酸对严重烧伤大鼠外周血Th／Ts比值及存活率的影响

Effects of dietary arginine on peripheral blood Th/Ts ratio and survival rate in severely burned rats

目的：探讨严重烧伤大鼠外周血Ｔｈ／Ｔｓ比值的变化及精氨酸对该变化的调节作用和对大鼠存活率的影响。方法：Ｗｉｓｔａｒ大鼠６０只，分为Ａ、Ｂ、Ｃ３组，Ａ、Ｂ组制作３０％体表面积（ＴＢＳＡ）Ⅲ度烧伤模型，伤后分别用生理盐水和精氨酸灌胃；Ｃ组给以假伤后用生理盐水灌胃，伤后１４ｄ内动态测定Ｔｈ／Ｔｓ比值并记录动物存活情况。结果：（１）烧伤后第３天Ｔｈ／Ｔｓ下降，第１０天下降最为明显。（２）精氨酸能防止烧伤后Ｔｈ／Ｔｓ比值下降，这一现象从伤后第５天起比较显著。（３）精氨酸能提高伤后动物的存活率。结论：严重烧伤后补充精氨酸是提高机体免疫力和存活率的有效治疗手段。

Objective:To study the effect of supplemental dietary arginine on dynamic changes of peripheral blood Th/Ts ratio and survival rate in burned rats.Methods:60 Wistar rats were divided into 3 groups .including 30% TBSA Ⅲ °thermal injury(group A,B) and false injury(group C).The animals were supplied with saline solution(group A,C) or arginine solution(group B).300 μl whole blood was sampled dynamically within 14 d to measure the ratio of Th/Ts.The number of survival rats was also recorded. Results:(1)Th/Ts ratio dropped at 3 d and had a valley point at 10 d postburn. (2)Arginine could prevent the falling of Th/Ts ratio, which was distinct at 5 d after burn. (3)Survival rate at 14 d postburn was raised in arginine group. Conclusion : Dietary arginine may be beneficial to severely burned patients.