胶原、甲壳胺和超氧化物歧化酶及其复合物对烧伤创面愈合的作用

Effects of collagen,chitosan and SOD and their compound on burn wound healing in mice

目的：观察胶原、甲壳胺和超氧化物歧化酶（ＳＯＤ）及其复合物对小鼠烧伤创面愈合的作用。方法：２０～２５ｇ的昆明小鼠４０只，分成４组，每组１０只，制成１５％体表面积（ＴＢＳＡ）Ⅱ度烧伤，创面分别外用生理盐水、１０％胶原、１．５％甲壳胺、胶原＋甲壳胺＋ＳＯＤ复合物（１：１＋１００Ｕ／ｍｌ）０．２ｍｌ，每天治疗一次，直至伤后１５ｄ取材，分别以酶反应法测定表皮细胞Ｎ－乙酰－β－Ｄ－氨基葡萄糖苷（ＮＡＧ）酶，流式细胞仪测定皮肤组织的ＤＮＡ含量和细胞周期。结果：（１）一定浓度的ＳＯＤ可使培养人表皮细胞形成较多和较大的细胞集落，干表皮细胞生长呈正相关的ＮＡＧ酶测定表明，９０Ｕ／ｍｌＳＯＤ时表皮细胞的ＮＡＧ酶量最大。（２）生理盐水，胶原，甲壳胺，胶原＋甲壳胺＋ＳＯＤ４种不同治疗方法的皮肤组织ＤＮＡ０含量测定表明，各治疗组的ＤＮＡ含量均高于对照组，尤以复合物组含量最高。（３）皮肤组织细胞周期分析表明，各治疗组Ｓ期和Ｇ２＋Ｍ期的细胞百分含量增多，也以复合物治疗组增加的量最为显著。结论：胶原、甲壳胺和ＳＯＤ复合物可从不同的方面促进烧伤创面愈合。

Objective:To observe the effects of collagen,chitosan and superoxide dismutase(SOD) and their compound on the topical treatment of burn wound in the mice.Methods:(1)Fourty mice weighing 20￣25 g were made into a 15% Ⅱ degree burn model and divided into four groups:10/0.i collagen.1.5%chitosan,collagen +chitosan +SOD(1:1+100 U/ml) and control group. After burn.10% collagen.1.5% chitosan,and the compound were spreaded onto the burn wound of the treatment group(0.2 ml/d.for 15 d).whereas saline(0.2 ml/d,for 15 d) in the control group.(2)The effect of SOD on epidermal cells was evaluated by NAG enzyme.(3) Cell cycle and the contents of DNA of biopsy specimens were evaluated by flow cytometry. Results:(1)The result of NAG emzyme detection of the epidermal cell indicated that SOD in suitable concentrations promoted the growth of epidermal cells.(2) The DNA contents were higher in the treatment groups , especially significant in the compound of collagen+chitosan+SOD group;(3) The percentages of S and G2+M phases were also higher in the treatment groups.especially significant in the compound group.Conclusion:The compound of collagen,chitosan and SOD can promote wound healing in different ways.