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免疫聚合酶链反应新技术在胃癌血清诊断中的应用 被引量:2

Tmmuno-PCR, a new technology in sero-diagnosis of gastric cancer
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摘要 应用本所建立的免疫聚合酶链反应技术(IMMUNO-PCR)检测胃癌患者血清中的肿瘤相关抗原MG7Ag,以探讨其在胃癌诊断中的价值。结果发现,在198例胃癌患者血清中MG7Ag阳性者164例,阳性率为82.8%,其他肿瘤患者的阳性结果分别为:食管癌(15/86),结肠癌(40/90),肝癌(0/84),卵巢癌(1/45),子宫癌(0/27),肺癌(4/66),在非癌患者的检测结果:溃疡病(6/78),慢性胃炎(7/118),慢性肠炎(2/60),正常献血员(2/236)。结果提示,免疫PCR技术对于胃癌包括部分早期胃癌的论断具有重要价值,同时也可能成为肿瘤普查时筛选高危人群的一种有用的工具。 A serum -marker of tumor associated antigen, MG7Ag, in gastric cancer by means of Immuno-PCR, had been detected recently in our Institute. It was positive in 164 out of 198 cases with gastric cancer (82.8%). The rates of positivity in other cancers were as follows: esophageal carcinoma (15/86), colonic carcinoma (40/90), liver cancers (0/84), ovarian cancer (1/45), uterine cancer (0/27), lung cancer (4/66). Those among benign diseases were peptic ulcer (6/78), chronic gastritis (7/118), chronic colitis (2/60), normal blood donor (2/26). It is suggested that the Immuno-PCR technique is of great value in the diagnosis of gastric cancer including early cancer. It is also a potential screening tool in mass servey of high risk population of gastric cancer.
出处 《中华消化杂志》 CAS CSCD 北大核心 1996年第S1期19-21,共3页 Chinese Journal of Digestion
基金 国家杰出青年科学基金资助课题
关键词 胃癌 单克隆抗体 聚合酶链反应 诊断 Diagnosis of Gastric cancer Monoclonal antibody Im-muno-polymerases chain reaction
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参考文献6

  • 1任军,樊代明,周绍娟,张学庸,杨安钢,李明峰,陈峥.免疫聚合酶链反应技术的建立及其对胃癌细胞上MG_7-Ag的检测[J].中华肿瘤杂志,1994,16(4):247-250. 被引量:20
  • 2樊代明 张学庸 等.抗低分化胃癌细胞系MKN-46-9单克隆抗体的制备及免疫组化鉴定[J].解放军医学杂志,1988,13(1):12-12.
  • 3Kohler,G,Milstein C.Continuous culture of fused cells secreting antibody of perdefined specificity. Nature . 1975
  • 4Saiki R K,Gelfand D.H,Stoffel S. J. et al.Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science . 1988
  • 5Ren J,Fan D. M Zhou S. J. et al.Molecular cloning of v, vk genes and sequencing of Vk genes from monoclonal antibody NGC-1 against gastric cancer. J. Gastroenterol & Hepatol . 1993
  • 6Sano T,Smith CL.Cantor CR,et al.Immuno-PCR:very sensitive antigen detection by means of specfic antibody-DNA conjugates. Science . 1992

二级参考文献4

  • 1Ren J,J Gastroenterol Hepatol,1993年,8卷,增刊,226页
  • 2刘学锋,国外医学遗传学分册,1992年,6卷,286页
  • 3樊代明,解放军医学杂志,1988年,13卷,12页
  • 4樊代明,中华消化杂志,1988年,8卷,25页

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