免疫聚合酶链反应新技术在胃癌血清诊断中的应用

Tmmuno-PCR, a new technology in sero-diagnosis of gastric cancer

应用本所建立的免疫聚合酶链反应技术(IMMUNO-PCR)检测胃癌患者血清中的肿瘤相关抗原MG7Ag,以探讨其在胃癌诊断中的价值。结果发现,在198例胃癌患者血清中MG7Ag阳性者164例,阳性率为82.8％,其他肿瘤患者的阳性结果分别为:食管癌(15/86),结肠癌(40/90),肝癌(0/84),卵巢癌(1/45),子宫癌(0/27),肺癌(4/66),在非癌患者的检测结果:溃疡病(6/78),慢性胃炎(7/118),慢性肠炎(2/60),正常献血员(2/236)。结果提示,免疫PCR技术对于胃癌包括部分早期胃癌的论断具有重要价值,同时也可能成为肿瘤普查时筛选高危人群的一种有用的工具。

A serum -marker of tumor associated antigen, MG7Ag, in gastric cancer by means of Immuno-PCR, had been detected recently in our Institute. It was positive in 164 out of 198 cases with gastric cancer (82.8%). The rates of positivity in other cancers were as follows: esophageal carcinoma (15/86), colonic carcinoma (40/90), liver cancers (0/84), ovarian cancer (1/45), uterine cancer (0/27), lung cancer (4/66). Those among benign diseases were peptic ulcer (6/78), chronic gastritis (7/118), chronic colitis (2/60), normal blood donor (2/26). It is suggested that the Immuno-PCR technique is of great value in the diagnosis of gastric cancer including early cancer. It is also a potential screening tool in mass servey of high risk population of gastric cancer.