肝癌S-100^+树突状细胞的免疫组织化学研究

Immunohistochemical study on S-100 protein-positive dendritic cells in patients with hepatocellular carcinoma

用ABC免疫酶标染色观察24例肝癌、15例肝硬化、9例慢性活动性乙型肝炎、14例慢性迁延性乙型肝炎,9例急性乙型肝炎及11例正常肝组织中S-100^+树突状细胞(DC)情况。结果:肝癌组DC数量较所有组明显增高(P<0.05);肝硬化组较除肝癌组以外所有组高(P<0.05);正常组较所有乙型肝炎组高(P<0.05),HBsAg阳性肝癌组较HBsAg阴性肝癌组高(P<0.05);肝癌组织中DC主要分布于癌细胞之间及淋巴细胞成簇区,DC的树突接触或环抱肿瘤细胞。上述结果提示:①DC参与机体抗肿瘤免疫反应;②DC在乙型肝炎病毒引起的慢性肝炎→肝硬化→肝癌演变过程中可能起一定预防作用。

The distribution and number of dendritic cells (DC) were studied by ABC immunohistochemical method in specimens of hepatocellular carcinoma (HCC, n=24), cirrhosis of liver (CL, n=15), chronic active hepatitis B (CAH, n=9), chronic persistent hepatitis (CPH, n=14), acute hepatitis B(AH, n=9) and normal liver (NL, n=11). The results showed that 1) in HCC, DC was mainly distributed in the intercellular space of the tumor cells and the clustered lymphoid cell area, the tumor cells were surrounded by the dendrites of DC; 2) the number of DC cells/mm2 in HCC (12.8) was significantly higher than that in CL (8.4); which was again significantly higher than that in NL (6.1); 3) the number of DC in NL was higher than those in CAH (3.9), CPH (3.3) and AH (2.9); and 4, the number of DC in HBsAgpositive HCC was higher than that in HBsAgnegative HCC. The above results suggested that 1) DC might be involved in the host immune reaction against tumor by means of antigen presentation; 2) DC might play some protective role in the evolution of CAH→CL→HCC.