抗癌药物与钙拮抗剂合用对体外人胃癌细胞的影响

Effect of combined use of chemotherapeutic agents and calcium antagonists on human gastric cancer cell lines in vitro

报道了抗癌药物5氟脲嘧啶(5FU)、阿霉素(DOX)及长春新碱(VCR)与钙拮抗剂维拉帕米(VPM)、尼卡地平(NIC)、汉防己甲素(TET)、硝苯吡啶(NIF)及硫氮(艹卓)酮(DIL)单独和联合应用对体外人胃癌细胞株MKN45、MKN28及MGC803细胞增殖影响的研究结果。结果发现,无毒剂量VPM或NIC与低浓度VCR、DOX及5FU合用时,可使这些化疗药物抑制胃癌细胞增殖的作用增强2.7～7.5倍。小剂量的TET可增强DOX对3种胃癌细胞株的细胞毒作用,亦可增强VCR及5FU对某些细胞株的作用。而NIF和DIL对抗癌药物细胞毒性的增强作用不显著。

The effects of anticancer drugs 5-fluorouracil(5-FU), doxorubicin (DOX) and vincristine (VCR) used either alone or in combination with calcium antagonists (CAs) as verapamil (VPM), nicardipine (NIC), tetrandrine (TET), nifedipine (NIF) and diltiazem (DIL) on the proliferation of human gastric cancer cell lines (MKN45, MKN28 and MGC803) were studied in vitro. In the experiments of combined use of cytotoxic drugs and CAs, for MKN45, MKN28 and MGC803 cells, the addition of VPM or NIC in non-toxic dosage and DOX, 5-FU and VCR in low concentrations could enhance the inhibitory effect on gastric cancer cell, proliferation to 2.7 to 7.5-folds. By addition of TET in low dosage, a significant potentiation of DOX cytotoxicity was found in all tumour cell lines, and the sensitivity of some tumor cells to VCR or 5-FU tended to be increased as well. NIF or DIL rarely significantly enhanced the cytotoxicity of these antineoplastic agents on 3 gastric cancer cell lines.