川芎对急性肾功能衰竭家兔肾组织中6-keto-PGF_(1α)、TXB_2含量影响

The Effect of Ligusticum Wallichii on Renal Tissue TXB_2,6-keto-PGF_(1a) and 6-keto-PGF_(1a)TXB_2 in Rabbits with Glycerol Induced Acute Renal Failure

本研究在以往实验基础上,用放射免疫测定法分别测定了甘油致家兔急性肾功能衰竭(ARF)后24、48及72h 肾脏皮质、髓质组织中6-keto-PGF_(1α)、TXB_2及 6-keto-PGF(1α)/TXB_2比值,发现 ARF 后72h 肾脏皮质组织中 TXB_2含量增高(P<0.05),6-keto-PGF_(1α)含量无明显变化(P>0.05),6-keto-PGF_(1α)/TXB_2比值降低(P<0.05),应用川芎防治ARF,能使肾皮质组织中6-keto-PGF_(1α)、TXB_2无明显变化,72h 时6-keto-PGF_(1α)/TXB_2比值仍能维持正常水平,而对肾脏髓质组织中上述指标无明显改变(P>0.05)。推测川芎对家兔 ARF 的防治作用可能是部分地通过抑制肾脏皮质组织中血小板激活,纠正 PGI_2/TXA_2平衡失调而发挥作用,而不是通过肾脏髓质组织起作用。

The effects of preventing and treating rabbits with glycerol-induced acute renal failure(ARF)with ligusticum wal- lichli(LW)were studied.Fourty two male and female rabbits weighing 2.0～3.0kg were divided into three groups ran- domly:(1)LW-treated group(18 rabbits),(2)pathological control group(18 rabbits),and(3)normal control group( 6 rabbits).6-keto-PGF_(1α),TXB_2 concentration and 6-keto-PGF_(1α)/TXB_2 ratio in renal tissue were measured with radio- immunoassay 24hr,48hr,and 72hr after the onset of ARF.The results showed that renal cortex TXB_2 concentration ob- viously increased(P<0.05),6-keto-PGF_(1α)concentration had no obvious changes(P>0.05),6-keto-PGF_(1α)/TXB_2 ratio markedly decreased(P<0.05);and LW could keep 6-keto-PGF_(1α)./TXB_2 ratio remaining in normal level for 72hr after ARF.There was no significant difference in the concentration of the above items of renal medulla between LW treated group and pathological control group.It was showed that preventing and treating effects of LW on ARF were produced partly by inhibiting the activation of renal cortex platelet and correcting 6-keto-PGF_(1α)/TXB_2 imbalance,not by the func- tions of renal medulla.