柴胡皂甙D对肾损害大鼠蛋白尿抑制作用的实验研究

Experimental Study on Inhibitory Effect of Saikosaponin D on Proteinuria in Injured Kidney Rats

本实验利用单克隆抗体1-22-3引起雌性 Wistar 大鼠肾损害模型,验证了柴胡皂甙 D(ss-D)经腹腔注射对蛋白尿具有明显的抑制作用。注射 ss-D0.2mg·kg^(-1)体重d^(-1)时,与磷酸盐缓冲液(PBS)对照组比较,第1天、3天尿蛋白结果有显著性差异(分别为 P<0.05和 P<0.01)。每日注射 ss-D0.6mg/kg 体重时,第1、3、5、8天尿蛋白结果与PBS 对照组比较,均有显著性差异,特别是第3天和第8天(P<0.001)。间接免疫荧光染色发现,经 ss-D 治疗后,肾小球中单核巨噬细胞的表面标识(TR-3,ED-1)和 T 淋巴细胞抑制细胞的表面标识(OX-8)阳性细胞数量明显降低,提示 ss-D 具有抗炎和免疫调节作用。

The animal model of renal lesions induced by monoclonal antibody(MoAb)1-22-3 was used to confirm the effect of the various amount of Saikosaponin D(SSD)on proteinuria.The optimal amount of SSD was realized to be 0.6mg/kg body weight.Especially on the third day,14.03±8.12 mg/24h urine vs 154.03±55.59 mg/24h urine compared with the control group(P<0.001).On the eighth day,16.63±8.11 mg/24h urine vs 69.31±22.04 mg/24h urine(P< 0.001)respectively.The significant decrease of amount of the surrogate marker of monocyte/macrophage in renal glomer- alus(TR-3 and ED-1)postitive cells and the surrogate marker of T lymphocyte suppressor(OX-8)positive cells in SSD group compared with that in phosphate buffer saline solution(PBS)group was demonstrated using indirect immusufluo- rescent stain.The results indicated that Saikosaponin D has the anti-inflammatory and immue regulatory effect.