60Coγ射线对簇毛麦愈伤组织细胞的分裂及染色体的影响

Effect of 60 Co γ Radiation on Calli of Haynaldia Villosa

为了探明６０Ｃｏγ射线对簇毛麦愈伤组织的细胞分裂及染色体变异的影响，采用１０－３００Ｇｙ剂量辐照，结果表明７０～３００Ｇｙ引起细胞的间期致死，３０－６０Ｇｙ引起分裂致死。１０－６０Ｇｙ均能引起染色体的结构及数量变异，其中有利于融合双方染色体整合或重组的片段化在６０Ｇｙ辐射后第３天频率最高。

In order to find out the influence of 60 Co γ ray on the cell division and chromosome aberration of Haynaldia villosa calli,doses from 10～300Gy were used for irradiation.The results showed that 70～300Gy induced interval lethality of cells and 30～60Gy division lethality.All the doses from 10 60 Gy could lead to the aberration of chromosome both in number and structure.The chromosome fragmentation which was beneficial to the recombination between the partner chromosomes occurred with the highest frequency at the third day after irradiation of 60 Gy.The radiation effect was also influenced by the duration time following subculture to irradiate.