用筐架式填充床生物反应器高密度培养工程细胞

High Density Engineering Cell Culture in a Packed Bed Bioreactor

用圆盘型聚酯载体筐架式填充床内作为细胞生长分裂的支持物，通过笼状唧筒生物反应器连续培养能合成和分泌ｒｔＰＡ的工程细胞，经２８ｄ连续灌注培养，细胞密度达４７×１０７／ｍｌ，ｒｔＰＡ浓度最高达９９μｇ／ｍｌ，ｒｔＰＡ活性最高达４０６８ＩＵ／ｍｌ。

A packed bed perfusion system for the production of high yielding culture is described employing a stationary basket with polyester fiber disks as a support matrix for immobilized cell. A caged pumping impeller is also empolyed for internal liquid recirculation. Studies were conducted in a Celligen Plus packed bed bioreactor with serum containing medium for the growth of rCHO cells and serum free medium for the production of t PA. After 28 days of perfusion culture in the packed bed system, a final density of 4.7×10 7 cells per cm 3 of bed volume was achieved. The highest density of rt PA was 9.9 μg/ml, and the activity of rt PA can be 4068 IU/ml. The process demonstrates that this system can increase the cell density and the production level of genetic product.