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牛碱性成纤维细胞生长因子在酵母系统中的表达 被引量：2

Expression of the Gene Coding for Bovine Basic Fibroblast Growth Factor in the Yeast Saccharomyces cerevisiae
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摘要 牛碱性成纤维细胞生长因子在酵母系统中的表达方向东陈淳谢志伟耿解萍王小宁戚正武（中国科学院上海生物化学研究所国家分子生物学实验室上海２０００３１）碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）是ＦＧＦ家族... The fibroblast growth factor(FGF) constitude a family of at least seven structurally related polypeptides, among them basic FGF(bFGF) is the most characterized. They play an important role in vivo in embryonic development, angiogenesis,nervous cell system differentiation ,and wound repair. According to the published cDNA sequences. The structural gene of bovine bFGF(bbFGF) was amplified by the PCR method, and constructed in the expression plasmid pVT102U/αˉbbFGF.The expressed product was purified by chromatography on CMˉ52 cellulose and affinity chromatography on heparin Sepharose 4B. The immunogenic property of the expressed product was detected using the ELISA method,and its bioactivity of stimulating effect on 3T3 cells was also mesured.They both showed the same activition with the natural one.
作者 方向东 陈淳 谢志伟 耿解萍 王小宁 戚正武
机构地区 中国科学院上海生物化学研究所国家分子生物学实验室
出处 《生物工程学报》 CAS CSCD 北大核心 1996年第S1期266-270,共5页 Chinese Journal of Biotechnology
关键词 BOVINE basic FIBROBLAST growth FACTORS bbFGF YEAST GENE expression Bovine basic fibroblast growth factors,bbFGF,yeast,gene expression
分类号 Q786 [生物学—分子生物学]
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  • 2Arnaud E, Touriol C, Boutonnet C, etal. A new 34-kilodalton isoform of human fibroblast growth factor 2 is cap dependently synthesized by using a non-AUG start codon and behaves as a survival factor [J]. Mol Cell Biol, 1999, 19(1) :505.
  • 3Quarto N, Finger F R, Rifkin D B. The NH2-terminal extension of high molecular weight hFGF is a nuclear targeting signal [J]. J Cell Physiol, 1991, 147(2):311.
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  • 7Cronauer M V, Schulz W A, Seifert HH, el al. Fibroblast growth factors and their receptors in urological cancers: basic research and clinical implications [J]. European Urology, 2003, 443(3):309.
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生物工程学报
1996年 第S1期

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