从大肠杆菌包含体中提取有活性的人溶菌酶的研究

The Studies on Recovery of Biologically Active Recombinant Human Lysozyme from Inclusion Bodies in Escherichia coli

从大肠杆菌包含体中提取有活性的人溶菌酶的研究钱世钧陈欣叶军郭良栋郭伟（中国科学院微生物研究所北京１０００８０）在医药和工业上，许多非常有应用前景的真核多肽，往往由于它们天然来源缺乏，不能充分提供产品，限制了它们的应用范围。通过基因克隆技术使其在大肠...

Biologically active human Iysozyme from an engineered bacterium E.coli was obtained by isolation and solubilization of inclusion bodies as well as renaturation of solubilized polypeptides. The inclusion bodies could also be obtained after the treatment of cell with acetone,instead of sonication.The additions of PEG, glutathione glycerol,or sodium sulfite and sodium thiosulfate in the renaturation solution, can increase yield of renatured active human lysozyme.