人纤溶酶原激活剂的抑制物2在昆虫细胞中的表达

Expression of Human Plasminogen Activator Inhibitor 2 in the Baculovirus Expression System

人纤溶酶原激活剂的抑制物２在昆虫细胞中的表达周爱武裴轶董雪吟谢维徐贤秀（南京大学生物化学系国家医药生物技术重点实验室南京２１００９３）纤溶酶原激活剂（ＰｌａｓｍｉｎｏｇｅｎＡｃｔｉｖａｔｏｒ，ＰＡ）对血液中蛋白水解酶的活性有重要的调节作用。纤溶酶原激...

A cDNA coding for human plasminogen activator inhibitor 2 (PAI 2) was first inserted into a transfer vector,pBlueBac,which could produce blue plaque for selection. Then it was transfered into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter by cotransfection.Cells infected with recombinant virus synthesized a 43kDa PAI 2 protein at a level of 15%～18% of total cellular protein. Intrestingly,the protein without a signal peptide can be secreted into the medium partially and accounts for 60% of the total protein in culture medium. The recombinant PAI 2 was identical to native PAI 2 purified from human placenta with respect to interaction with polyclonal antibody and inhibition of urokinase type plasminogen activator.