固定化犁头霉菌在含有丙二醇的体系中转化RSA为皮质醇

Conversion of RSA into Hydrocortisone by Immobilized Absidia orchidis in a Propanediol containing System

固定化犁头霉菌在含有丙二醇的体系中转化ＲＳＡ为皮质醇王俊陈长治李保安张金红俞跃庭（南开大学分子生物学研究所天津３０００７１）皮质醇是重要的甾体激素类药物之一，目前国内采用淡紫色犁头霉菌（Ａｂｓｉｄｉａｏｒｃｈｉｄｉｓ）催化１１脱氧皮质醇醋酸酯（Ｒ...

In order to raise the yield of hydrocortisone from RSA, Absidia orchidis was immobilized with calcium alginate gel, and the conversion was conducted in a 1,2 propanediol containing system. Experimental results showed that the β/α ratio of the product was 2 3～2 5 and the yield of β isomer was reached to 60% in this paper comparing with β/α of 0 9～1 2 and β isomer yield of 45%～50% in the aqueous solution by free Absidia orchidis . When Tween80 was added to the reaction system, the β isomer yield was increased remarkably. The optimum pH value was 5 8 and the optimum conversion temperature was 28℃.