酶在有机相中催化酚类聚合的研究

Phenolic Polymerization Catalyzed by Peroxidase in Organic Media

酶在有机相中催化酚类聚合的研究徐越平黄国兰俞耀庭（南开大学分子生物学研究所天津３０００７１）（南开大学生物活性材料开放实验室天津３０００７１）酶曾一度被认为只能在水介质中起催化作用，而有机溶剂则会使其失活。由于大量化学反应都是在有机溶剂中进行的，...

Phenolic polymerization was carried out by enzymatic catalysis in organic media. Phenols and aromatic amines with electron with drawing groups could hardly be polymerized by HRP catalysis,while phenols and aromatic amines with electronˉdonating groups could easily be polymerized. The reaction rate of either the paraˉsubstituted substrate of metaˉsubstituted substrate was higher than that of orthoˉsubstituted substrate. The reaction rate increased with the increase of reaction temperature from 14 to 40℃,but decreased when temperature exceeded 40℃.Horseradish peroxidase and lactoperoxidase could easily catalyze the polymerization,but chloroperoxidase and laccsae failed to yield polymers.Metallic ions such as Mn 2+ ,Fe 2+ ,and Cu 2+ could poison horseradish peroxidase to various extent.