云南红豆杉愈伤组织培养及其生产紫杉醇的研究

Studies on Callus Culture and Its Taxol Pruduction of Taxus yunnunensis

云南红豆杉愈伤组织培养及其生产紫杉醇的研究甘烦远彭丽萍郑光植（中国科学院昆明植物研究所昆明６５０２０４）从红豆杉科红豆杉属植物如短叶红豆杉（Ｔａｘｕｓｂｒｅｖｉｆｏｌｉａ）等的树皮或枝叶提取到的紫杉醇（Ｔａｘｏｌ）是一种具有强抗癌活性的二萜烯类化合...

Callus could be induced from twigs and needles from Taxus yunanensis . Synthesis for taxol in callus was analyzed by HPLC method. A callus strain with stable growth rate and taxol yield was selected by successively subcultured and habituated for above 10 generations. Its growth rate and taxol content had reached 0.30 g/L.d and 0.014% respectively, Some regulating conditions including different medium, different kinds of hormones and their concentrations, different organ addictives and others were studied. From them, the culture condition and culture system which was more suit for callus was set up. Also, the most suitable harvest time of callus was found by investigated the growth charge of callus and the systhesis process of taxol.