L缬氨酸对环孢菌素形成的影响

Ｌ缬氨酸对环孢菌素形成的影响赵德修（中国科学院植物研究所北京１０００４４）环孢菌素的生物合成路线受添加外源氨基酸影响。Ｋｏｂｅｌ和Ｔｒａｂｅｒ［１］报道过，添加与环孢菌素形成有关的特殊氨基酸可以提高环孢菌素某组分所占的比例。如ＤＬ氨基丁酸能够提高...

Externally supplemented L valine enhanced yield of cyclosporin A by 27 times and slighly inhibited formation of cyclosporin B,C, and G in synthetic medium. This amino acid was not among variable effecting the growth rate of the culture. L valine appear attractive for evolving feeding strategies that could lead to increase of final cyclosporin A titer. In contrast, effect of L valine on production of cyclosporin A is more than of DL valine. ACKNOWLEDGEMENT I wish to express my deep sense of gratude to Dr. Zdenek Rehacek, DSc., my Ph.D. Supervisor, Head of Laboratory of Metabolism of Microbial Eukarytes and Director of the Course UNESCO ROSTE, for his able guidance and continuos willingness to discuss, argue about, and constructively criticizing this work. My sincere thanks are extended to Dr. M. Flieger and Ing. M. Beran for their concern, cooperation and valuable help in this work.