原位PCR对胃癌和胃炎组织内幽门螺旋杆菌的检测

Detection of Helicobacter pylori in Gastritis and Gastrical Cancer Samples by in situ PCR

为探讨幽门螺旋杆菌(Helicobacter pylori, Hpy)与胃癌发病的关系,我们建立了原位(PCR(in situ PCR,ISPCR)方法,并使用它对胃炎和胃癌组织内的幽门螺旋杆菌进行了检测。发现在胃炎组织内Hpy的感染率为83.3％,胃癌组织内的感染率为65％。在11例胃癌阳性标本中,4例为细胞核阳性,2例细胞浆阳性,5例为所带正常胃腺组织内阳性。提示,Hpy的致癌机理,除与细菌的长期刺激外,尚可能与其参与细胞的功能调控有关。

This investigation examined the correlation between Hehcobacter pylori (hpy) infection and the risk of gastric cancer, used in situ FCR (ISPCR). The prevalence of Hpy infection. 83. 3% , was observed in patients with gastritis and 65% in patients with cancer. Among 11 positive gastric carcinoma samples. 4 cases positive position was in nuclea. 2 cases in cytoplasma and 5 cases in normal gastric mucosa. The results indicated that Hpy may have an important role in gastric carcmogenesis.