摘要
应用抗HCV NS4 McAb以酶标链霉亲和素生物素化二抗方法(LAB法),对IFN_2治疗前后肝组织进行免疫组化研究。结果显示HCV-Ag位于肝细胞浆中,治疗前后肝内HCV-Ag定位相似。但是治疗后HCV-Ag阳性肝细胞数量有明显变化,IFN有效者HCV-Ag阳性肝细胞数量明显减少或消失,且与血清HCV-RNA的变化基本一致。治疗前后肝HCV-Ag染色的变化与肝HAI计分变化的符合程度优于血清ALT和HCV-RNA。提示肝内HCV-Ag的检测可能有助于IFN疗效的预测和评价。
To observe the changes in hepatitis C virus NS4 antigen in liver tissue before andafter IFN therapy. Methods: the liver tissue from 9 patients treated with IFN-α were studied immunohistochemically using LAB method of HCV NS4 McAb. Results: HCV-Ag was present in the cytoplasm of hepatocyte. The pattern of HCV-Ag staining in post-treatment liver samples was the same as those in the pretreatment liver samples. But the amount of HCV-Ag positive hepatocytes showed a significant difference. Patients who had a benificial response to IFN-a had significantly less HCV-Ag staining in liver tissue after treatment than before. The pattern of HCV-Ag staining was more compatible with the changes of histological activity index than the change of serum ALT or HCV-RNA. Conclusion: The examination of hepatic HCV-Ag may be useful in predicting and assessing the response to INF-α therapy.
出处
《胃肠病学》
1996年第1期14-16,共3页
Chinese Journal of Gastroenterology
