急性SIVmac感染猴模型评价中药1号体内抗病毒效果

EVALUATING ANTIVIRAL EFFECT OF CHINESE TRADITORAL MEDICINCE IN THE PROCRESS OF ACUTE SIVmac MONKEY MODEL

采用ＳＩＶｍａｃ感染剂量（１～１０ＭＩＤ５０）和先给药后感染方式，中药Ⅰ号能１００％保护动物不发生活动性ＳＩＶ感染，表现在感染治疗后从血浆分离病毒阴性，无可检出的抗体，ＰＣＲ检测ＰＢＭＣＳＩＶｍａｃＤＮＡ阴性，但巢式—ＰＣＲ检测２／３猴阳性。对照组３／４猴建立了真正感染，病毒分离阳性，抗体上升。中ＳＩＶｍａｃ感染剂量（１０～１００ＭＩＤ５０和先感染后给药方式，中药Ⅰ号未能抑制感染后２周的血浆病毒水平，但与对照组比较明显降低感染２周后的血浆病毒水平。表明中药Ⅰ号有抗猴体内病毒的作用。本模型亦可应用于中药方剂猴体内抗病毒的效果评价。

All Rhesus monkeys were preserved from infection by pre-infection administrated orally Chinese medicine NO.11(CM-1),then they were inoculated intravenously with low infected for of SIVmac(1～10MID_(50)) .No sign of SIVmac infection appeared, neither isolation of SIVmac from plasma nor antibody were negative. By means of PCR to examine PBMC SIVmac DVIA showed negative ,but by nested PCR two of three monkeys positive. In contrast, three of four in the control group monkeys were infected, isolation of SIVmac from plasma positive, antibody increased. Using the moderate infected for of SIVmac (10～100MID(50)) inoculated intravenously and then adminstrated CM-1 orally, viremia appeared two weeks affer infection. However the CM-1 could decreased the level of viremia when if compered with the control group monkeys.The data showed that CM-1 could decrease the number of virus in vivo. The acute SIVmac infected monkeys model can be used to evaluate the effects of anti-viral drug including Chinese traditional Medicine.