PHA及rhIL－2对新鲜分离肝癌细胞体外实验观察

Experiment Study on the Freshly Isolated Liver Cancer Cells Using PhA and RHIL-2 Culture in Vitro

本文从本院外科８例肝脏病人，合并肝门静脉高压行肝叶切除的肝组织，分离培养肝癌细胞。其中４例见肝癌细胞生长。对其中１例新鲜分离肝癌细胞中加入植物血凝素（ＰＨＡ）及重组人白细胞介素－２（ｒｈＩＬ－２），培养３天后发现，原来成片成堆生长的肝癌细胞逐渐减少，细胞结构破坏，７－９天后在细胞消失，周围淋巴细胞增多。未加ＰＦＡ及ｒｈＩＬ－２３例肝癌细胞生长旺盛，而周围淋巴细胞却逐渐减少消失。提示：ＰＨＡ及，ｒｈＩＬ－２对肝癌细胞周围淋巴细胞有刺激生长作用，对自体肝癌细胞有抑制作用。

ight cases of surgical hepatectomy tissue culture in vitro:5 cases of primary carcinoma of theliver,3 cases of hepatocirrhosis anid one case of liver hydatid disease All cases were complicated byhepatoportril hypertensive,In the 4 cases specimens with liver cancer cells growth,the diagnosesof clinical and pathologic biopsy conformed to primary liver carcinoma. We investgated the effectof phytohemagglutinin(PHA)and recomhinant human interluekin-2(rhIL-2)on the in vitrogrowth of freshly isolated liver cancer cells from one of the speciment with liver cancer cellgrowth,using PHA stimulated previously and then rhIL-2 activated the tumor infiltratinglymphcyte(TIL),it showed that liver cancer cells growth decreased after 3 days of culture andafter 7-9days almostly disappeared,however,TIL increase .Our study deonstrate the combinantionof PHA and rhIL-2 with liver cancer cells growth in vitro has stimulating effect on TIL and aspecific reduction effect on autoliver cancer cells.