补肾固齿丸对大鼠实验性牙周炎牙槽骨重建的影响

Effects of Bushen Guchi Bolus on alveolar bone reconstruction of periodontitis rats

目的研究补肾固齿丸对大鼠实验性牙周炎牙槽骨重建的影响,进一步探讨补肾固齿丸对牙周炎的治疗机制。方法成功建立大鼠牙周炎模型之后,大鼠分为正常组、牙周炎模型组、补肾固齿丸组,给予补肾固齿丸治疗,采用放射免疫法测定血清骨钙蛋白与Ⅰ型胶原交联C端肽浓度,同时采用显微CT对大鼠牙槽骨进行三维重建,以评价补肾固齿丸对大鼠实验性牙周炎牙槽骨修复重建的影响。结果经过补肾固齿丸治疗1个月后,补肾固齿丸组血清骨钙蛋白与Ⅰ型胶原交联C端肽浓度[分别为(0.859 1±0.156 9)、(2.038 6±0.407 5)]较牙周炎模型组[分别为(1.806 4±0.258 5)、(3.056 0±0.461 5)]下降(P<0.05)。补肾固齿丸组大鼠牙槽骨的骨小梁体积分数(0.304 1±0.010 0)明显高于牙周炎模型组[(0.181 6±0.020 0),P<0.05],且骨小梁数量(3.124 4±0.100 0)略高于牙周炎模型组[(2.088 9±0.200 0),P>0.05],而补肾固齿丸组的骨小梁分离度(0.575 1±0.020 0)较牙周炎模型组(0.611 0±0.030 0)有降低的趋势(P<0.05)。结论补肾固齿丸能改善牙周炎大鼠牙槽骨代谢状况,促进牙槽骨改建,对牙周炎具有一定的辅助治疗作用。

Objective To explore the influence of Bushen Guchi Bolus on the alveolar bone reconstruction of rats with experimental periodontitis,and to investigate the treatment mechanism of the drug.Methods The model of experimental periodontitis were established by means of steel wire,high-carbohydrate diet and periopathogens inoculation in rats.Then Bushen Guchi Bolus was given to rats of treatment group at a dose of 0.8 g/（kg·d） by gastric injection for 30 d,and 10 ml/（kg·d） normal saline was give to rats of normal control and model group for same period.All rats were killed at then end of treatment.The contents of osteocalcin（OCN） and pyridinoline cross-linked carboxy-terminal telopeptide of type Ⅰ collagen（ICTP） in the serum were observed by radioimmunoassay（RIA）.Micro-CT and software digitized specimens were reconstructed three-dimensionally for parameter assessment of alveolar bone（including bone volume /total volume,trabecular number,and trabecular separation）.Results Rat experimental periodontitis models were successfully established by steel wire,high-carbohydrate diet and periopathogens inoculation.After 30 days＇ treatment of Bushen Guchi Bolus,the level of OCN and ICTP in the serum were decreased in treatment group（0.859 1±0.156 9 and 2.038 6±0.407 5,P0.05）,compared with model control group（1.806 4±0.258 5 and 3.056 0±0.461 5）.After treatment,BV/TV of alveolar bone had distinct improvement（0.304 1±0.010 0,P0.05）,and Tb.N showed a trend of increase in the treatment group（3.124 4±0.100 0,P0.05）.In addition,Tb.Sp in the treatment group（0.575 1±0.020 0） was inferior to that of the model group（0.611 0±0.030 00,P0.05）.Conclusion Bushen Guchi Bolus improves alveolar bone reconstruction of rats with experimental periodontitis.