GUS和HPT基因的基因枪法转化水稻

The Biolistic Transformation of Rice with GUS and HPT Gene

以水稻（丹粳－５号）愈伤组织为受体材料，采用基因枪法将含有ＧＵＳ和ＨＰＴ基因的ＣＭ２质粒导入水稻细胞．经过５０ｍｇ／Ｌ的潮霉素筛选，获得抗性愈伤组织，并在同样筛选压力下诱导分化成苗，获得抗性苗．共获得１７个抗性克隆．ＧＵＳ组织化学检测表明，有１２个克隆为ＧＵＳ阳性，ＧＵＳ和ＨＰＴ基因共表达率为７０％左右．Ｓｏｕｔｈｅｒｎ分子检测证明ＨＰＴ基因已整合到水稻基因组中．

The plasmid CM2 that contains both GUS gene and HPT gene driven byCaMV 355 promoter was introduced into rice callus using the biolistic method.The hygromycin resistant calli were obtained and regenerated into plantlets underthe selective pressure of 50mg/L hygromycin. 12 out to 17 resistant clones showedGUS activities. The frequency of co-expression of GUS and HPT genes are approximately 70%. The integration of HPT gene into rice genome was confirmedby Southern Hybridization analysis.