球孢白僵菌对七种蚜虫的感染反应——时间-剂量-死亡率模型分析

Susceptibility of Seven Species of Aphids to a Beauveria Bassiana Isolate ——Analysis of Time-Dose-Mortality Model

通过以球孢白僵菌（Beauveria bassiana）菌株SGBB8601系列浓度的分生孢子悬液（104～108孢子/mL）对俄罗斯麦双尾蚜（Diuraphis noxia）、麦无网长管蚜（Metopolophium dirhodum）、麦长管蚜（Sitobion avenae）、麦二叉蚜（Schizaphis graminum）、玉米蚜（Rhopalosiphum maidis）、禾谷缢管蚜（R padi）和忽布疣额蚜（Phorodon humuli）等七种蚜虫的体表接种试验,运用时间-剂量-死亡率模型分析了该菌株作用于上述寄主的剂量、时间及其互作效应。所测菌株对所有蚜虫均具有毒力,接种后七日的LD50为1.25×105～114.82×105孢子/mL,毒力因蚜种而异,双尾蚜和忽布疣额蚜最为敏感,玉米蚜与禾谷缢管蚜的敏感性相对较低,其余居间。这些结果表明所试菌株具有作为蚜虫微生物防治候选因子的潜势。本研究采用的分析方法较传统的Probit或Logit分析方法有显著的优越性,将时间和剂量效应的分析统一到一个模型中,使时间与剂量效应的互作评价成为可能。笔者介绍了该模型的生物学基础和建模步骤,并讨论了在生物学研究领域的应用前景。

A time-dose-mortality model was used to analyze data from bioassaying of a Beauveria bassiana isolate (SGBB8601) at gradient doses in the suspension of 104-108 conidia/mL topically applied to seven species of crop aphids, including the Russian wheat aphid Diuraphis noxia , the rose-grass aphid Metopolophium dirhodum, the English grain aphid Sitobion avenae, the greenbug Schizaphis graminum, the corn leaf aphid Rhopalosipum maidis, the bird-cherry oat aphid R. padi, and the hop aphid Phorodon humuli. The model was found to well fit the data and generated more useful information than probit or logit analysis conventionally used. The significant advantage of the model over the conventional method was that the dose and time effects and their interaction could be displayed in a single model. Based on the parameters estimated by fitting the model, cumulative and conditional mortality probabilities were given for each aphid species under the influence of the B. bassiana isolate. For the aphid species tested, the isolate had LD50's varying with time and LT50's varying with dose. The estimates of LD50 one week after treatment ranged from 1. 25× 105 to 114. 82× 105 conidia/mL with D. noxia and P. hunuli being most susceptible and R. padi and R. maidis lest susceptible. The results showed high potential of the isolate for use in microbial control of aphids.