摘要
The neurons with coexistence of serotonin-and giutamate-immunoreactivity(5-HT/Glu cells)in medullary raphe nuclei were investigated in the present study by using immunofluorescence technique.On the basis or the investigation projections of the 5-HT/Glu cells to the cerebellar cortex were studied by means or a combined microsphere retrograde transport.The results showed that most of the 5-HT cells in medullary raphe nuclei exhibited Glu-immunoreactivity.The cell count indicated that 81.77% of the 5-HT cells contained Gin-immunoreactivity;40.35%of the Gin-cells contained 5-HT.The retrograde labeled cells by microspkere(MS cells)were found in all medullary rapke nuclei. 5-HT/MS cells were rare.The Gin/MS cells had a rate in total MS cells(40.31 %).Three trible-labeled cells (5-HT/Glu/MS cells)were found. The finding or the 5-HT/Glu cells in medullary raphe nuclei projecting to the cerebellar cortex may give an iportant significance to understanding the functional connection between medullary raphe nuclei and cerebellum.
The neurons with coexistence of serotonin-and giutamate-immunoreactivity(5-HT/Glu cells)in medullary raphe nuclei were investigated in the present study by using immunofluorescence technique.On the basis or the investigation projections of the 5-HT/Glu cells to the cerebellar cortex were studied by means or a combined microsphere retrograde transport.The results showed that most of the 5-HT cells in medullary raphe nuclei exhibited Glu-immunoreactivity.The cell count indicated that 81.77% of the 5-HT cells contained Gin-immunoreactivity;40.35%of the Gin-cells contained 5-HT.The retrograde labeled cells by microspkere(MS cells)were found in all medullary rapke nuclei. 5-HT/MS cells were rare.The Gin/MS cells had a rate in total MS cells(40.31 %).Three trible-labeled cells (5-HT/Glu/MS cells)were found. The finding or the 5-HT/Glu cells in medullary raphe nuclei projecting to the cerebellar cortex may give an iportant significance to understanding the functional connection between medullary raphe nuclei and cerebellum.
