红细胞电泳时间测定影响因素的探讨

FACTORS INFLUENCING RED BLOOD CELL ELECTROPHORESIS TIME (RET)

探讨了 ＲＥＴ测定中的四项影响因素，发现三种电泳介质（０．９％蔗糖、０．１４５ｍｏｌＮａＣｌ、自身血浆），六种离子浓度（０．１４５～０．１７０ｍｏｌ／ＬＮａＣｌ）和三种实验温度（２５℃、３７℃、４３℃）的ＲＥＴ值差异非常显著（Ｐ＜０．００５），十种细胞浓度（１．０～１０．０ × １０~９／Ｌ）的 ＲＥＴ值差异不显著（Ｐ＞０．５００～０．７５０）．

Four factors affecting the results of RET have been observed. With 3 different electrophoresis media(those were 0. 9% cane sugar solution,0. 145mol/L sodium chloride and auto -plasma). 6 different ionic strengths of the salt bridge (concentrations ranging from 0. 145 -0. 170mol/L),and 3 experimental temperaturs(25℃ .37℃ ,43℃ ) . the differences in the RET were statistically significant(P < 0. 005). There were,however, no significant differences in RET results among 10 different cell concentrations (P > 0. 500 - 0.75).