摘要
本文采用荧光偏振法，测得血液高粘滞组红细胞膜微粘度（４．０１０± ０．２８６）显著高于正常对照组（３．４４５± ０．１７２）．证实血液物理状态与细胞膜微观流变性质密切相关．用ＩＦＮ—γ与血液高粘滞组红细胞温育，膜微粘度显著下降（Ｐ＜０．００１），表明膜流动性受外界生物活性大分子影响明显．
The membrane microviscosity of erythrocytes was determined by fluerescence polarization analysis using the Shimadzu RF - 540 fluorespecthophtometer with X - lamp off. The patients with high blood viscosity (HBV)had increased membrane microviscosity of erythrocytes as compared with the control group(P < 0.001). It was agreed with data obtained in rats. After 4OU/ml IFN - r had acted on erythrocytes of HBV patients at 37? for half an hour,the memberane microviscosity decreased significantly. The results indicated that the moderation of IFN - γ on membrane fluidity may be the main factor that IFN - r actted on the body.
出处
《中国血液流变学杂志》
CAS
1996年第1期5-8,共4页
Chinese Journal of Hemorheology
关键词
荧光偏振
血液流变学
IFN—γ
膜脂区
流动性
fluorescence polarization analysis
hemorheology
membrane fluidity
interferon -γ
