肺癌P53蛋白免疫组织化学研究

P53 Protein Expre s sion in Lung Cancer with Immunohistochemical Method

为了探讨P53基因突变在肺癌发生,发展过程中的作用及其与肺癌临床。病理指标之间的关系,本文用免疫组织化学方法,对164例各病理类型肺癌,用抗P53蛋白的单克隆抗体(DO—7)进行了P53蛋白表达的检测。结果发现:100例出现了P53蛋白阳性表达,阳性率为60.90％,其中鳞状细胞癌、腺癌、大细胞癌、小细胞癌的阳性表达率分别为75.27％(47/72)、70.45(31/44)、57.14％(4/7)43.9％(18/41),各病理类型之间阳性率无显著性差异(P>0.05),表明P53基因的改变与相当比例的肺癌有关,与其病理类型无明显关系,进一步分析发现P53蛋白表达与肺癌组织学分级、局部淋巴结转移、临床分期及预后之间无明显关系(P>0.05),而与患者是否吸烟有关(P<0.05),与鳞状细胞癌关系更为明显(P>0.05)。这一结果间接提示P53基因突变可能发生在肺癌癌变过程的较早期,因而可能成为肺癌早期,因而可能成为肺癌早期诊断和临床监测的重要标志物。

The experssion of P53 protein was examined in 164 cases of lung cancer with immunohistochemcal techique in which a monocional antibody (DO-7) specific to P53 protein was used, and the relationship between expression of P53 protein and some of clinical and pathological parameters was analyzed. All of the results showed that P53 protein expression occured in 60.98% of lung cancer and the absence or presence of P53 immnostaining correlated with smoking habits of patients with lung tumor ( P<0.05 ) , especially with squamous cell carcinomas ( P<0.01 ) , but did not with pathological types. histological grading. clinical staging, lymph node metastasis and prognosis ( P>0.05 ) .This results suggest that P53 gene mutation may occur in the earlier stages of progression, of pulmonary cancer and P53 protein experssion may become an important marker of early diagnosis and clinical observation of lung cancer.