高效离子交换色谱检测冻融交替处理对蛋白质的影响

The Effect of Temperature on Protein Change Detected by Ion-Exchange High Performance Liquid Chromatography

将各种温度处理的标准蛋白质进行了阴离子交换高效液相层析，结果表明，冻融交替并常温放置长时间，以及水溶解或特定ｐＨ缓冲溶液溶解经贮藏一定时间，会导致肌蛋白降解或发生变化．

Using anion exchange chromatography column MonoQ, the structure change underdifferent temperature are compared, the result showed that myoglobin stored in water orpiperazine(pH5. 7) at─20℃, after freezing-thawing, at thawing interval 8 hours. The proteinbreak down more easily than store ambiently or freez-thawing alone.