双（6－氧－间－羧基苯磺酰基）β－CD·Fe^（3＋）模拟过氧化物酶的酶促反应动力学

Catalytic Kinetics Studies on Ferric Coordination Compound with Bis(6-Oxygen-m-Carboxyl Benzenesulfonyl)β-Cyclodextrin Used as An Artificial Peroxide

模拟过氧化物酶，采用愈创木酚法，求得了双６－氧－间－羧基苯磺酰基β－ＣＤ·Ｆｅ~３＋配合物（以下简称“模拟酶”）的酶促反应动力学曲线，所得曲线符合米氏方程（Ｍｉｃｈａｃｌｉｓ－ＭｅｎｔｅｎＥｑｕａ－ｔｉｏｎ）；采取米氏方程的双倒数方程（ＬｉｎｅｗｅａｖｅｒＢｕｒｋＥｑｕａｔｉｏｎ）作图，求得了模拟酶的特征常数Ｋｍ值（米氏常数）；在反应中，发现此模拟酶对底物Ｈ２Ｏ２具有较大亲合力，形成了“酶－底”复合物，由此求得模拟酶的反应机制．在相同实验条件下，分别测了两个参照物：ＦｅＣｌ３和双６－氧－间－羧基苯磺酰基β－ＣＤ对此反应的催化能力，作用不太明显．

The ferric coordination compound of bis6-oxygen-m-carbonyl benzenesulfonyβ-cyclodextrin which was a new synthesized compound characterized by 1H- 13C NMR, IRspectra and elemental analysis previously, could be used as an artificial peroxide to catalyseoxidoreduction between H2O2 and guaiacol efficiently under the conditions of room temperature and normal atmospheric pressure in the range of pH2. 5-3. 2 The catalytic curve wasfitted to the law of Michaelis-Menten kinetics. The apparent Km value was 4. 76 ×10-2mmol.L-1 H2O2 in the solution containing 30mmol.L-1 artficial enzyme and 0.1 mmol. L-1guaiacol at optimum pH2. 8. And in the range of temperature 25-90℃, rising temperaturemade the catalytic activity of the artificial enzyme increase rapidly and Q10 was 2-3 in rangeof temperature 25-60℃.