华北地区肝细胞癌中抑癌基因P＿（53）的突变

Mutations of the p53 Tumor Suppressor Gene in Hepatocellular Carcinoma from North China

华北地区属黄曲霉毒素低含量区，而乙肝病毒感染则普遍，本实验对该地区２４例石蜡包埋人肝癌和癌旁组织的Ｐ５３蛋白（突变型）进行了免疫组化（ＡＢＣ法）检测。结果显示３３．３％（８／２４）肝癌及其癌旁组织为阳性。说明肝癌抑癌基因Ｐ５３的突变不仅仅发生在黄曲霉毒素高含量区病人，其可能与乙肝病毒感染有关。

mmunohistochemical techniqe(ABC)was used to detect the expression of P53 protein(mutant type)in paraffinembedded tissues of human hepatocellular carcinoma(HCC)and corresponding non─tumor Liver(NT)from 24 pa-tients in North China,where aflatoxin levels in the diet are lower and chronic HBV infection are common.the resultshowed that the positive rate was 33.3%(8/24)in HCC and NT.this observation srggest that mutations of the tumorsuppressor gene P53 in HCC are not limited to patients from geographic regions where the ingestion of aflatoxin ishigher and may be associated with HBV infection.