鼻咽癌患者血清中抗重组EB病毒胸腺嘧啶激酶IgA抗体含量变化的初步观察

Observation of IgA against recombinant EBVThymidine kinase in sera of patients with na-sopharyngeal carcinoma

将含有ＥＢ病毒ＤＮＡ中编码胸腺嘧啶激酶（ＴＫ）的基因片段重组到棒状病毒ＤＮＡ上，再感染蛾卵细胞株ＳＦ－９，可获得大量纯度较高的重组ＴＫ（ｒＴＫ）表达。以此为抗原作碱性磷酸酶ＥＬＩＳＡ检测血清中抗ＴＫ的ＩｇＡ抗体（ＴＫ／ＩｇＡ）。对７０例鼻咽癌患者２５０例非鼻咽癌供血者（包括１９６例正常人、４９例慢性鼻咽炎和５例其它鼻咽疾病）作了ＩｇＡ／ＴＫ检测，并与ＩｇＡ／ＶＣＡ检测结果作对比，证实鼻咽癌患者血清ＩｇＡ／ＴＫ平均相对吸光度值（０．６３７６±０．２０２６）明显高于非鼻咽癌病人（０．３９４３±０．１６４６）或慢性鼻咽炎病人（０．３７３０±０．１５４３），其差别具统计学意义（Ｐ＜０．０００１）。同时检测ＩｇＡ／ＴＫ和ＩｇＡ／ＶＣＡ的鼻咽癌血清样品５４例，两法结果没有相关性（Ｒ＝０．１５０４，Ｐ＝０．２７７５。在鼻咽癌组中，两法没有统计学上的差异，而在非鼻咽癌组（包括一部分正常人）和慢性鼻咽炎线，则ＩｇＡ／ＴＫ的ＥＬＩＳＡ检测有较高的似然比，提示在鼻咽癌普查工作中，ＩｇＡ／ＴＫ检测对ＩｇＡ／ＶＣＡ阳性对象似乎较能排除鼻咽癌患病的可能性，从而缩小划分高危人群的范围，无疑能大大减少普查工作量，且ＥＬＩＳＡ检测方法简便，?

pstein一Barr virus(EBV)is associated closly with nasopharyngeal carcinoma(NPC)which is a common malignenttumor with a high incidence in Southern China. IgA/VCA assay is a common method for screening a high risk popula-tion of NPC. Thymidine kinase(TK)is well known as a member of four replication-related enzymeactivties in cells in-fected by EBV. Purified antigen(rTK )was obtained from SF-9 cell line which was infected by Baculovirus recombinat-ed with EBV DNA fragment coding Thymidine kinase and then ground by supersonic. After covering plastic culture96 wells plate with rTK antigen at 4C overnight the sera diluted l :20 was added before adding antibody Labelledwith Alkaline phosphatasc(AP) against human IgA. Relative optical density was read by the reader after staining,us-ing anti-IgA-AP diluted 1:4000 and substratc reaction as a standard control(OD=1).Results of the ELISA showedthat absorbtion for rTK in patients with nasopharyngeal carcinoma(0.6336±0.1758)was obviously higher than thatin normal and chronic nasopharyngitis donors(0.3673± 0.1561,0.3730±0.1543,4espectively,P<0.000l ),whichsuggested that rTK ELISA be useful for diagnosis of NPC. Meanwhile IgA/VCA as done and compared with rTKELISA. In NPC group there were not significant differences between positive ratc of IgA/VCA andrTK ELISA, butin non-NPC and normal group that of IgA/VCA was higher than that of rTK ELISA. For the conclusion.the authorssupposed that the results is related to NPC. but we need more practice to improve the method.