基因扩增法检测肺炎衣原体DNA的研究

Dctection of chlamydia pneumoniae by polymcrase chain reaction

本研究采用聚合酶链式反应（PCR），建立检测肺炎衣原体DNA的实验诊断方法。结果显示，采用肺炎衣原体PstⅠ编码区的两对特异性引物（HL－1－HR－1和HM－1-HR－1）进行扩增，两株肺炎衣原体（TW－183和CM－1）均可见437bp和229bp的特异性扩增带，而沙眼衣原体和鹦鹉热衣原体扩增结果为阴性。检测敏感性为100fg左右。该研究技术的建立，为肺炎衣原体的早期诊断和流行病学调查提供了一种快速、敏感、特异的新方法。

We have developed a method of detection of Chlamydia pneumoniae, based upon the polymerase chain reaction (PCR). A cloned C. pneumoniae 474bp Pst Ⅰ fragment was sequenced, and two specific restrietion fragments were chosen as primers for use in PCR. The results showed that two strains of C. pneumoniae (TW-183 and CM-1) tested were amplified by the HL-1-HR-1 primer pair or the HM-1-HR-1 primer pair,producing the expected 437 bp and 229 bp amplification products respectively. None of the chlamydia trachomatis serovars,chlamydia psittaci strains, or other organisms tested were arnplified. Bands of positive amplified DNA were detected in 100 fg template DNA. Our study suggested that this technique could be applied to clinical specimens for rapid identifiction and specific diagnosis of chlamydia pneumoniae infection.