晶体后囊混浊防治的实验研究

Laboratory Investigation in Prevention and Treatment of Posterior Capsule Opacification.

后囊混浊是影响白内障术后视功能的常见并发症。本文对正常及白内障晶体上皮细胞进行体外培训,观察体外培养的晶体上皮细胞的生物学以及组织学变化。应用5-氟脲嘧啶,高三尖杉酯碱及骆驼蓬碱对组织培养的晶体上皮细胞进行药物抑制试验。结果显示人类晶体上皮细胞在体外生存能力有限,体外的生存能力与供体的年龄成负相关。高三尖杉酯碱及骆驼蓬喊可有效地抑制晶体上皮细胞的生长繁殖,同时根据培养的晶体上皮细胞生长特性的观察,作者建议使用边缘光滑的撕囊术,以降低白内障术后后囊混浊的发生率。

Posterior capsule opacification is a common complication resulting from posterior migration and proliferation of the lens epithelial cells after extracapsular cataract extraction. The human lens epithelial cell culture was established in vitro in normal and cataractous lenses. The cytobiological features and the histological characterization of the cultured human lens epithelial cells were investigated. The Homoharringtonice(HH) and Harmaline were used to inhibit the proliferation of the human lens epithelial cells in vitro. The results revealed that the proliferation capacity of the cultured human lens epithelial cells was reversely proportional to the donour age. Compared to the 5-Fu,the HH and harmaline had the effection to inhibit the cultured lens epithelial cells,but the toxicity to the ocular tissuer required further investigation. It is suggested from the results that the small and smooth edged anterior capsulohexis in the ECCE could decrease the posterior capsule opacification.