rIL-2激活的骨髓细胞抗瘤活性——白血病自体骨髓移植净化

ANTITUMOR EFFECT OF rIL-2-ACTIVATED BONE MARROW CELLS——PURGING RESIDUAL LEUKEMIC CELLS IN ABMT

用rIL-2激活未分离的骨髓外胞(bone marrow cell,BMC)观察其抗瘤活性和造血功能。经rIL-2激活24h后的未分离BMC即表现对NK细胞敏感的K562和NK细胞不敏感的Raji细胞均有杀伤活性;在一定时间内,随激活时间延长抗瘤活性增强,在第4天达高峰。rIL-2激活的未分离BMC与分离BMC二者抗瘤活性无差异。5例急性非淋巴细胞白血病病人骨髓经rIL-2激活后,白血病祖细胞CFU-L生成能力下降;经rIL-2激活的骨髓细胞生成CFU-GM能力无下降。根据我们的研究结果提出,rIL-2激活未分离BMC可用于白血病ABMT中残留白血病细胞净化,其方法简便、实用。

Antitumor effect and hematopoietic function of non-separated bone marrow (nBM) cells activated with recombinant interleukin 2 (rIL-2) were studied. The nBM cells activated with rIL-2 for 24 hours were cytotoxic against both NK-sensitive K562 and NK-resistant Raji cells. Within a limited time, the longer the bone marrow cells activated, the higher the antitumor effect appeared and a climax was reached on the fourth day. There was no difference between nBM and separated BM cells activated with rIL-2 in their antitumor effect. Leukemic stem cells' ability to form CFU-L decreased progressively in 5 acute myelogenous leukemia patients, along with the prolongation of time of activation of BM cells with rIL?. The nBM cells7 ability to form CFU-GM showed no difference in rIL-2 and non-rIL-2 culture medium. In 5 patients with leukemia treated by autologous bone marrow transplantation (ABMT), the nBM cells were purged with rIL-2 for 48-72 hours in vitro and hematopoietic reconstruction was not affected post-ABMT. According to these results, it is concluded that the nBM cells activated with rIL-2 can be used to purge the residual leukemic cells and at the same time hematopoietic reconstruction is not affected. This method is simple and practical.