绵羊胚胎移植受体同期化处理技术研究

Study on Synchronization Treatment of Embryo Transfer in Ewes

采用ＰＧ注射、ＭＡＰ口服或明道理植以及与不同促性腺激素和释放激素配合对受体母羊进行同期化处理，共分三次试验１１组１９种处理。０．０５ｍｇＰＧ间隔１１天两次阴唇注射；４５ｍｇＭＡＰ阴道埋植１０天，撤栓同时注射ＰＧ０．０５ｍｇ，ＰＭＳＧ３００ＩＵ，ＬＲＨ—Ａ３１００单位，４８ｈ注射ＬＨ２０单位以及埋植ＭＡＰ４５ｍｇ１０天，撤栓同时注射ＰＧ０．０５ｍｇ，ＰＭＳＧ３００ＩＵ，第１３天注射ＬＲＨ－Ａ３３００单位、ＬＨ１０单位，第１１～１３天每日两次注射ＦＳＨ５０×２和２０×２。受体母羊处理后７２ｈ发情同期化、排卵母羊率、卵巢黄体率分别为１００％、１００％．１００％；８３％、１００％、１００％和且６６％、１１６％、２０Ｏ％、供一受体同期时间差零天、－１、＋１天的移植妊娠率分别为５０％、１００％、１００％；１００％、５０％、１００％和０、０、０，受体总移植妊娠率分别为５０％、７５％和７５％，两个对照组以上指标分别为９０％、１００％，１００％、１１３％，５０％、６０％，７５％、７５％，５０％、５０％和５０、０％。结果表明受体同期化处理明显提高了移植妊娠率（Ｐ＜０．０５）。

This research was designed to synchronization treatment embryo transfer recipients ewes by injection PGF2a, MAP per os, gonadotropic and GnRH, following experiments 3, 11 groups and 19 treatments. Experiments showed: injection PGF2a in twice 0.05mg interval 11 days, at labium ;intravaginal sponge implant for 10 days, at the same withdraw, injection PGF2a 0. 05mg, PMSG 300IU, LRHA, 100 ug, after 48 h injection LH 20mg; implant MAP 45 mg for 10 Days, repeal sponge simultaneity ynjection PGF2a 0. 05mg,PMSG 300IU, 13th day LRH-A, 300ug, LH 10mg 11-13th day for FSH 50 ×2 and 20× 2 twice per day. The result showed: recipients of ewes synchronous estrus rate in 72 h, ration ovulation ewes, rate of ovary corpus luteun, respectively were 100, 100, 100%; 83, 100,100% and 166, 116,200%. Deficit in estrus time 0.-1, +1 day pregnancy rate of teansfer separately were 50, 100, 100%: 100, 50, 100 %: 0, 0,0 %; total pregnancy rate of recipients respectively were 50, 75 and 75%. Above aim in 2 control groups respectively90, 100; 100 113; 50, 60 ; 75, 75; 50, 50; and 50, 0%. The study prove synchronization treatment improve rate gestation of recipients (P<0. 05).