哈特口服液防治大鼠心脏缺血再灌注损伤及抗凝血作用的研究

Hate Oral Liquor(哈特口服溶液)in Preventing and Treating Myocardial Ischemlc/Reperfusion Injury in Rat Hearts and Anticoagulant Action

在大白鼠离体工作心脏正常灌注时,哈特口服液(哈特)显著升高左心室收缩压、左心室内压最大变化速率(P<0.01),增加心输出量(P<0.01),降低左室舒张末期收缩压(P<0.05),轻度增加冠状动脉流量。冠状动脉结扎后,哈特能防治心功能指标的急骤下降。再灌注初期哈特组心律失常的发生率为37.50％,与硝苯啶和对照组(均为100％)比较,有显著性差异(P<0.01)。随着再灌注的延续,哈特和硝苯啶促进心脏的收缩和舒张功能以及心输出量的恢复。哈特能减少缺血再灌注心肌组织丙二醛的生成(P<0.05)。提示哈特能防治心肌缺血再灌注损伤。同时还观察到哈特有降低血小板聚集性和延长凝血时间的作用。

The effects of Hate oral liquor(HOL)on ischemia reperfusion injury have been compared with those of nifedipine and control in the isolated rat hearts using the hemodynamic study.HOL obviously increased LVSP. ±dp/dtmax and cardiac output(P<0.01),and lowered LVEDP(P<0.05),and slightly raised the coronary flow in the nonischemic hearts.The hearts underwent 15 min ischemia induced by ligation of coronary artery and 25 min reperfusion.HOL showed significant preservation of contractility and relaxation during ischemia.From the moment up to 2 rain of reflow,the incidence of arrhythmia was 37.50% in HOL,compared with 100% in both nifedipine and control(P<0.01).HOL and nifedipine promoted the recoveries of heart functional parame- ters and cardiac output gradually.During the period of reperfusion,the content of myocardial malondialdehyde (MDA)was lower in HOL than that in nifedipine and control(P<0.05).The results demonstrated that HOL could prevent and treat myocardial ischemic reperfusion injury.HOL could also inhibit platelet aggregation and prolong coagulation time.