理气开郁方药对猫睡眠节律电活动调节作用的实验研究

Experimental Study of Modulation of Liqi Kaiyu Recipe(理气开郁方)on Brain Electrical Activities of Sleep in Cats

以猫的眼球运动电图(EOG)、大脑皮层电图(EEG)、肌电图(EMG)和外侧膝状体放电(PG0)为电生理学指标,连续记录6h,依据其电变化,将睡眠—觉醒周期分为清醒期(W),慢波睡眠Ⅰ期(SWS Ⅰ)、慢波睡眠Ⅱ期(SWS Ⅱ)和快动眼睡眠期(REM),观察理气开郁复方注射液及其单方柴胡皂甙注射液和郁金二酮注射液对猫睡眠节律电活动的调制。结果发现这些注射液能使猫的睡眠时间延长,尤以SWSA Ⅱ明显;柴胡皂甙注射液和郁金二酮注射液还能使 REM 明显延长。

The Liqi Kaiyu (LQKY) recipe regulates the flow of Qi and removes obstruction.The effect of LQKY,in- jection Saikosides and tulipine on sleep (N-REM stage Ⅰ,N-REM stage Ⅱ and REM or PS) was investigated in cats elector physiological recordings of EEG,EOG,ENG and PGO,they were studied for six consecutive hours. Results:(1) Injection LQKY could prolong SWS-Ⅱ time by 90.2% (P<0.01);(2) Injection saikosides (saiko- sides plus volatile oil) could prolong SWS-Ⅱ time by 77.6% (P<0.01) and total sleeping time by 49.9% (P< 0.05);(3) Pure extract of saikoside seems to be more effective than that of compound preparations.It could lengthen SWS-Ⅱ time by 119.5%.REM time by 100.8% and total sleeping time by 87.4%(P<0.01);(4) Tulipine injection could prolong SWS-Ⅱ time by 89.8%.REM time by 97.6% and total sleeping time by 68.1% (P<0.01).