温州蜜柑起源新探——同工酶证据

Studies on the Origin of Satsuma Mandarin by Isozyme Analysis

利用聚丙烯酰胺凝胶电泳法,分析了49个宽皮柑桔生物型的过氧化物酶、谷氨酸—草酰乙酸转氨酶、6—磷酸葡萄糖异构酶、6—磷酸葡萄糖变位酶、苹果酸酶、超氧化物歧化酶、四唑氧化酶以及酯酶等8种酶系统同工酶。运用数量分类学的原理及方法,对同工酶资料进行了类平均聚类分析。根据酶谱及聚类分析结果,认为温州蜜柑来源于黄岩本地广桔的实生变异。

The leaf extracts of 49 biotypes of mandarin were analyzed by polyacrylamide gel electrophoresis for isozymes of peroxidase, glutamate oxaloacetate transaminase, phosphoglucose isomerase, phosphoglucose mutase, malic enzyme, superoxide dismutase, tetrazolium oxidase and esterase. Dissimilarities of the isozyme patterns among the samples were calculated by computer based on the UPGMA method to demonstrate the phylogenetic relationships of the samples. Based on the isozyme analysis, it can be concluded that satsuma mandarin (Citrus unshiu Marc. ) may originate from the nucellar mutation of 'Huangyanbendiguangju' man-darin(C. nobilis Lour. ).