摘要
用酶联免疫吸附试验(EnzymeltinkcdImmunosorbrntAssay ELISA)和蛋白质免疫印迹技术(westermblot).对番木瓜环斑病毒外壳蛋白(PRSV-CP)基因转基因植株进行表达蛋白的检测。ELISA的结果表明,外壳蛋白基因已在转基因番木瓜中表达;westernblot分析结果表明,外壳蛋白基因在转基因植株中的表达产物具有与天然蛋白同样的大小及相同的血清学反应。
Papaya-ringspot-virus cocqt-prot ein(PRSV-CP)expressed in transgenic Papaya plantlets wasdetected by tising Enzyme Linked Immunosorbent Assay(ELISA)cqnd Western blot.ELISA detection showed expression of PRSV-CP gene in the transgenic plantlets.Western blot analysis dis-closed that the gene prodticts expressed in transgenic Papaya had a molecular weight(MW)andserological reaction similar to natural viral coat protein。
出处
《热带作物学报》
CSCD
1995年第S1期36-39,共4页
Chinese Journal of Tropical Crops
基金
国家"八五"攻关项目