甘蔗钙调蛋白基因的克隆及序列分析

CLONING AND SEQUENCING OF A cDNA SEQUENCE ENCODING CALMODULIN FROM SUGARCANE

从甘蔗（ＳａｃｃｈａｒｕｍｓｐｅｃｉｃｓｈｙｂｒｉｄＣＶ．）茎顶端分生组织分离总ＲＮＡ，经反转录合成ｃＤ－ＮＡ第一条链，以此为模板进行多聚酶链式反应（ＰｏｌｙｍｃｒａｓｅＣｈａｉｎＲｃａｃｔｉｏｎ，ＰＣＲ），产物为一长为４５０ｂｐ的ＤＮＡ片段。经克隆及序列测定表明，其核苷酸序列及所推导的氨基酸序列，与其他植物的钙调蛋白（ＣａｌｍｅｄｕｌｉｎＣａＭ）基因的核苷酸序列及所编码的氢基酸序列，具很高的同源性。

The first strand of cDNA from the total RNA was extracted from stem apical generating tis-sue of sugarcane(Saccharum species hybrid,cv)plantlet and was used as a template for PCR re-action with oligonucleotide as a primer synthesized。ccording to the extremities of calmodulin genefrom barley。The PCR products were cloned into and sequenced.A very high ho-mology was found in calmodulin cDNA sequence and amino acid sequence deduced from the cDNAbetween sugarcane and other plants。