摘要
利用带有Ｔｒｐ启动子的大肠杆菌表达载体ＷＣＣ８能有效地表达西红柿Ｃｕ／ＺｎＳＯＤ基因，该表达蛋白在菌体中具有活性，并在一定程度上抑制受体菌ＳＯＤ基因的表达。据ＳＤＳ－ＰＡＧＥ分析，表达ＳＯＤ蛋白占茵体总蛋白２０％左右，其活性达３．０×１０５Ｖ／Ｌ发酵液。还利用ＮＢＴ法对表达ＳＯＤ蛋白的活性进行了研究。
The CV/Zn SOD(superoxide dismutase)gene of Tomato has been successfully cloned and ex-pressed in E. coli under the transcriptional control of the Trp promoters. The expression protein ofSOD gene prOduced was as much as 20%of total cellular protein by SDS-pAGE eletrophoresisand it showed activity in the host ceIls of E. coli by NBT method,while the expression of SODgene of the host cells was greatly inhibited.It was demonstrated that 3.0 × 105 U activity of SODcould be obtained from 1 LB medium after fermentation for 10h.
出处
《热带作物学报》
CSCD
1995年第S1期83-88,共6页
Chinese Journal of Tropical Crops
