植物及其病原的电镜样品制备研究

Studies on Preparation the Specimen of Electron Microscope for Plant and Plant Pathogen

应用健康烟叶组织，感染赤星病的烟叶组织．玉米缺素组织以及植物病原病毒烟草花叶病毒（ＴＭＶ）、黄瓜花叶病毒（ＣＭＶ）、马铃薯病毒Ｙ（ＰＶＹ）、马铃薯病毒Ｘ（ＰＶＸ）、香石竹班驳病毒（ＣａｒＭＶ）等和植物病原细菌ＸａｎｔｈｏｍｏｎａｓＯｒｙｚａｅ．病原真菌ＰｉｒｙｃｕｌａｒｉａＯｒｙｚｄｅ等材料，试验了超薄切片法、负染法及免疫电镜等方法制样观察，探索出上述植物及其病原的电镜样品制备的工作条件，与文献报道的方法相比略有简化和改进．

Tested negative stain,ultrathin section and Immuno Electron Microscopy(IEM)etc.that methods of preparation specimen of Electron Microscope(EM)by usingthe following materials:healty tissue of tobacco leaf,tissue of tobacco leaf infectedAlternaria alternata,tissue of maize lacked element,and the pathogen of plant virusessuch as Tobacco Mosaic Virus(TMV),Cucuinber Mosaic Virus(CMV),Potato VirusY(PVY),Potato Virus X(PVX),Carnation Mottle Virus(CarMV ect.,and thepathogenic bacteria of plant sucli as Xanthomonas Oryzae,the pathogenic fungus of plantsuch as Pirycularia Orvzae etc.,found out the working condition of the preparation spec-imen of EM for aforementioned plant and plant pathogen,and it has simplified and im-proved in comparison with the methods of the literature reported.