摘要
根据黄淮冬麦区小麦生态类型及栽培品种的特点,从降低SO_4^--、NO_3^-入手,设计研制了癸培养基.采用L_(16)(4~5)正交试验筛选出优良配方.经多年试验,癸培养基的愈伤组织平均诱导率分别较C_(17)、W_(14)、N_6培养基高1.3、2.06、2.42个百分点;并对各种不同的基因型都有广泛的适应性,且有促进愈伤组织形成早的显著特点.
Kui medium was developed, basically by reducing the concentration of SO4 and NO3- ,based on the biotypes and genotypes of wheat cultivars in Huang-Huai winter wheat areas. An appropriate composition of the medium was selected by orthogonal design L16(45). The frequencies of callus induction were increased by 1. 3> 2. 06 and 2. 42 points in percentage on average respectively when kui medium was used , compared with C17, W14, and N6 media. The results showed that kui medium promoted the initiation of callus formation significantly and suited for various genotypes.
出处
《华北农学报》
CSCD
北大核心
1995年第S1期47-50,共4页
Acta Agriculturae Boreali-Sinica
关键词
小麦
花药培养
癸培养基
诱导率
Wheat
Anther culture
Kui medium
Callus induction
