摘要
对石刁柏组织培养中生根难、发根慢、畸形很多的原因及其影响因子进行了研究.结果表明,根系诱导与材料的异质性、外植体生长状况、激素浓度及配比以及继代繁殖代数等条件关系密切.同一品种茎端直接生根较愈伤组织根诱导率高,正常根数亦多.2～3cm长的粗壮幼芽最宜作根诱导材料,细弱幼芽则须进一步复壮.适宜生根培养基为1/2MS+VB_10.3mg/L+IBA2.5mg/L+BA0.25mg/L,生根率为86%,平均根数达9.26条,畸形根率仅8%.继代次数宜控制在16代内.
Some primary factors causing hard and slow rooting and high abnormal root percentage in tissue culture of Asparagus officinalis L. were studied. Results indicated that rooting induction was closely related to the heterogeneity of materials, the growth state of explant, growth regulators and their concentrations,and generations of subculture,etc. In a same variety, rooting directly from apexes showed a higher rate than that from callus. A similar trend was observed in the case of normal root percent. The healthy bud lengthening 2-3cm is optimum for inducing, while a weak one needs rejuvenating before induction. The medium suitable for rooting is 1/2MS+VB10.3mg/L+IBA 2. 5mg/L+BA 0. 25mg/L,which results in rooting rate as high as 86% (the mean root number 9. 26)and reduces the abnormal root to 8%. It is best that the subculture is confined within 16 generations.
出处
《华北农学报》
CSCD
北大核心
1995年第S1期51-54,共4页
Acta Agriculturae Boreali-Sinica
关键词
石刁柏
组织培养
根诱导
畸形根
Asparagus officinalis L.
Tissue culture
Rooting induction
Abnormal roots
