高粱、玉米籽粒蛋白质含量直接蒸馏测定法的研究

Studies on Estimating Protein Contents of Sorghum and Maize Grains by Direct Distillation Method

根据每种作物籽粒蛋白质含量与酰胺含量之间存在着一定相关关系的原理,经反复试验,摸索出高梁和玉米籽粒蛋白质含量直接蒸馏测定法(简称DD法).该法省略了凯氏法的消化过程,把每个样品的测定时间由2h缩短为4min,测定结果的准确性、重现性与凯氏法测定结果比较无显著差异,既节省了时间和药品,又避免有毒气体对人体的危害,对大批量快速筛选作物育种材料十分有利.

Based on the principles of the relationship between protein and acylamino contents of different cereal grains,through repeated experiments,a direct distillation method(DD Method) is found in estimating protein contents of sorghum and maize grains. This method leaves out the digestion procedure in Kjeldahl's method,so it can cut short the testing time from 2 hours to 4 minutes for each sample. The accuracy and reappearance of DD Method has no significant difference in comparing with the result of Kjeldahl's Method. Therefore, this method can save much time and chemicals, and avoid toxic gases . It is favourable for screening large amount samples of breeding materials.