缺氧脑微血管内皮细胞的脂质过氧化与764－3的效应

Lipid peroxidation of endothelial cells of calf cerebral vessel subjected to hypoxia and its drug preservation

观察在缺氧脑微血管内皮细胞培养液中，丙二醛（ＭＤＡ）及乳酸脱氢酶（ＬＤＨ）的含量变化与７６４－３对这一变化的效应。发现缺氧２４小时脑微血管内皮细胞培养液中ＭＤＡ含量有显著性升高（Ｐ＜０．０１），经７６４－３处理后缺氧的内皮细胞培养液中ＭＤＡ含量处于正常水平。结果提示，７６４－３有抗氧化作用，但ＬＤＨ在上述条件下无显著性变化。

Abstract Endothelial cells isolated from the calf cerebral homogenates were cultivated in saturated oxygen(95%O2+5%CO2)chamber.The MDA and LDH contents in the supernatants of the cell culture wer determined in normal and hypoxic(93%N+5%CO2+2%O2) condition for 24 hours.The MDA content of the hypoxic group was markedly increased to 11.72±2.95 pmol/L in comparing to the normal group of 6.78±2.25pmol/L.It indicated that lipid peroxidation had been taken place during hypoxia.Presence of 764-3(an effective component of Chinese herb Salvia miltiorrhiza in hypoxic culture could restore the production of MDA to the normal level of control group.There was no sign of more LDH release after the hypoxic insult.It suggested that hypoxia can induce lipid peroxidation in cerebral vessel endothelial cells and 764-3 is a potent antioxidant in this case.